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114 p2a bjun pfast  (Addgene inc)


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    Structured Review

    Addgene inc 114 p2a bjun pfast
    114 P2a Bjun Pfast, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/114+p2a+bjun+pfast/pAAV-TRE-mRuby2+(Plasmid+%2399114)/pmc12307597-277-9-36
    Average 92 stars, based on 5 article reviews
    114 p2a bjun pfast - by Bioz Stars, 2026-09
    92/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Design and evaluation of a tripartite chemogenetic fluorescent reporter for visualizing ternary protein complexes
    Article Snippet: The plasmid pAG1225bis allowing the mammalian expression of Tom20-FKBP-pFAST 115–125 was generated by replacing the sequence encoding ECFP-FRB by the FKBP-pFAST 115–125 sequence, amplified from pAG1151, in the Addgene vector #171461 Tom20-CR coding for Tom20-ECFP-FRB . .. The plasmid pAG1713 allowing the mammalian expression of H2B-pFAST 99-114 -P2A-bJun-pFAST 1-98 -IRES-HA-mTurquoise2 was constructed by adding H2B sequence to pAG1256 (CMV pFAST 99-114 -P2A-bJun-pFAST 1-98 -IRES-mTurquoise2, which was itself constructed by adding bJun sequence from Addgene vector #22012 pBiFC-bJunVN173 to pAG1184) for expression of a fusion between H2B and pFAST 99–114 . .. The plasmid pAG1258 allowing the mammalian expression of myc-bFos-pFAST 115-125 -IRES-HA-iRFP670 was constructed by replacing FKBP sequence from pAG1151 by bFos sequence from Addgene vector #22013 pBiFC-bFosVC155.

    Expressing:

    Article Title: Design and evaluation of a tripartite chemogenetic fluorescent reporter for visualizing ternary protein complexes
    Article Snippet: The plasmid pAG1225bis allowing the mammalian expression of Tom20-FKBP-pFAST 115–125 was generated by replacing the sequence encoding ECFP-FRB by the FKBP-pFAST 115–125 sequence, amplified from pAG1151, in the Addgene vector #171461 Tom20-CR coding for Tom20-ECFP-FRB . .. The plasmid pAG1713 allowing the mammalian expression of H2B-pFAST 99-114 -P2A-bJun-pFAST 1-98 -IRES-HA-mTurquoise2 was constructed by adding H2B sequence to pAG1256 (CMV pFAST 99-114 -P2A-bJun-pFAST 1-98 -IRES-mTurquoise2, which was itself constructed by adding bJun sequence from Addgene vector #22012 pBiFC-bJunVN173 to pAG1184) for expression of a fusion between H2B and pFAST 99–114 . .. The plasmid pAG1258 allowing the mammalian expression of myc-bFos-pFAST 115-125 -IRES-HA-iRFP670 was constructed by replacing FKBP sequence from pAG1151 by bFos sequence from Addgene vector #22013 pBiFC-bFosVC155.

    Construct:

    Article Title: Design and evaluation of a tripartite chemogenetic fluorescent reporter for visualizing ternary protein complexes
    Article Snippet: The plasmid pAG1225bis allowing the mammalian expression of Tom20-FKBP-pFAST 115–125 was generated by replacing the sequence encoding ECFP-FRB by the FKBP-pFAST 115–125 sequence, amplified from pAG1151, in the Addgene vector #171461 Tom20-CR coding for Tom20-ECFP-FRB . .. The plasmid pAG1713 allowing the mammalian expression of H2B-pFAST 99-114 -P2A-bJun-pFAST 1-98 -IRES-HA-mTurquoise2 was constructed by adding H2B sequence to pAG1256 (CMV pFAST 99-114 -P2A-bJun-pFAST 1-98 -IRES-mTurquoise2, which was itself constructed by adding bJun sequence from Addgene vector #22012 pBiFC-bJunVN173 to pAG1184) for expression of a fusion between H2B and pFAST 99–114 . .. The plasmid pAG1258 allowing the mammalian expression of myc-bFos-pFAST 115-125 -IRES-HA-iRFP670 was constructed by replacing FKBP sequence from pAG1151 by bFos sequence from Addgene vector #22013 pBiFC-bFosVC155.

    Sequencing:

    Article Title: Design and evaluation of a tripartite chemogenetic fluorescent reporter for visualizing ternary protein complexes
    Article Snippet: The plasmid pAG1225bis allowing the mammalian expression of Tom20-FKBP-pFAST 115–125 was generated by replacing the sequence encoding ECFP-FRB by the FKBP-pFAST 115–125 sequence, amplified from pAG1151, in the Addgene vector #171461 Tom20-CR coding for Tom20-ECFP-FRB . .. The plasmid pAG1713 allowing the mammalian expression of H2B-pFAST 99-114 -P2A-bJun-pFAST 1-98 -IRES-HA-mTurquoise2 was constructed by adding H2B sequence to pAG1256 (CMV pFAST 99-114 -P2A-bJun-pFAST 1-98 -IRES-mTurquoise2, which was itself constructed by adding bJun sequence from Addgene vector #22012 pBiFC-bJunVN173 to pAG1184) for expression of a fusion between H2B and pFAST 99–114 . .. The plasmid pAG1258 allowing the mammalian expression of myc-bFos-pFAST 115-125 -IRES-HA-iRFP670 was constructed by replacing FKBP sequence from pAG1151 by bFos sequence from Addgene vector #22013 pBiFC-bFosVC155.



    Similar Products

    114 p2a bjun pfast  (Addgene inc)
    92
    Addgene inc 114 p2a bjun pfast
    114 P2a Bjun Pfast, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/114+p2a+bjun+pfast/pAAV-TRE-mRuby2+(Plasmid+%2399114)/pmc12307597-277-9-36
    Average 92 stars, based on 1 article reviews
    114 p2a bjun pfast - by Bioz Stars, 2026-09
    92/100 stars
    		  Buy from Supplier

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